Golnaz Taasoli; Farrokh Kafilzadeh; Darab Ghadimi; Juan Loor; Micheal Ballou
Volume 22, Issue 4 , December 2020, , Pages 529-536
Abstract
The objective of this experiment was to study the effect of diet containing high (40.7 %) non fibrous carbohydrates on some inflammatory and native immunity related genes expression in lactating Jersey cows. Seven lactating multiparous (2.5 ± 0.5) Jersey cows with 71 ± 3 days in milk ...
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The objective of this experiment was to study the effect of diet containing high (40.7 %) non fibrous carbohydrates on some inflammatory and native immunity related genes expression in lactating Jersey cows. Seven lactating multiparous (2.5 ± 0.5) Jersey cows with 71 ± 3 days in milk and 28 ± 6.6 kg of average milk production were used. All cows received a control diet containing low (34.3 percentage of dry matter) non fibrous carbohydrates content for the first 14 days and then were were switched to a diet containing high (40.7 percentage of dry matter) non fibrous carbohydrates content for one week. Blood samples were collected on day 0 (After 14 days of feeding control diet) and days 4 and 7 after feeding high NFC diet. Relative gene expression of seven genes (IL1-β, TNF-α, MNDA, CD74, TLR2, SELECTIN, TGF1-β) were measured. The results showed that gene expression of the pro-inflammatory cytokines (IL1-β and TNF-α) and anti-inflammatory proteins (MNDA, D74, TLR2, SELECTIN, TGF1-β) was down regulated during feeding of high NFC diet (p < 0.05). These data indicated that feeding a high non-fibrous carbohydrates diet caused inflammation and affected immunity system in lactating Jersey cows.